人急性胰腺炎早期外周血單核細胞Toll樣受體4表達變化_《中國普外基礎與臨床雜志》

作者：

李紅光 ¹ , 周總光 ¹ , 雷松 ² , 朱林 ³ , 鄭雪蓮 ¹ , 張鴻彥 ³ , 王永 ¹ , 張建成 ¹

1.四川大學華西醫院普外三科（成都 610041）;;
2.四川大學華西醫院腫瘤生物治療國家重點實驗室;;
3.四川大學華西醫院中西醫結合科;

關鍵詞：

急性胰腺炎硫酸乙酰肝素 Toll樣受體4 先天免疫

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的探討人急性胰腺炎早期外周血單核細胞(peripheral blood monocytes,PBMCs)表面Toll樣受體4(Toll like receptor 4,TLR4)表達變化。
方法發病24 h內入院的早期急性胰腺炎患者30例，采集入院當天及第3、7 d外周血及20名正常志愿者外周血，分離單核細胞。用流式細胞儀檢測單核細胞表面TLR4、CD14表達變化情況。同時檢測血清腫瘤壞死因子-α（tumor necrosis factor alpha,TNF-α）、白細胞介素6（interleukin 6,IL-6）等指標，研究它們之間的相關性。
結果急性胰腺炎患者PBMCs表面TLR4發病后表達上調， 1周左右恢復正常。TNF-α的變化與TLR4變化一致。
結論人急性胰腺炎早期可能通過先天性免疫門戶蛋白TLR4激活單核巨噬細胞系統導致TNF-α等促炎細胞因子的產生和釋放。

引用本文： 李紅光,周總光,雷松,朱林,鄭雪蓮,張鴻彥,王永,張建成. 人急性胰腺炎早期外周血單核細胞Toll樣受體4表達變化. 中國普外基礎與臨床雜志, 2006, 13(2): 197-200. doi: 復制

1.	[Ｊ].Science, 2002; 296(5566)∶ 301.
2.
3.
4.
5.	[Ｊ]. J Immunol， 2004; 172(1)∶20.
6.
7.	[Ｊ]. Arch Surg, 1993; 128(5)∶586.
8.
9.	[Ｊ]. J Surg Res, 1996; 63(1)∶369.
10.	[Ｊ]. Am J Respir Crit Care Med, 2003; 168(2)∶148.
11.	[Ｊ].Dig Dis Sci, 1997; 42(8)∶1783.
12.
13.	[Ｊ].Gastroenterology, 1997; 113(5)∶1741.
14.	[Ｊ]. Surgery, 2004; 135(4)∶376.
15.	[Ｊ]. Circulation, 2002; 105(6)∶685.
16.	[Ｊ]. Pancreas, 2005; 30(4)∶375.
17.	[Ｊ]. Eur J Surg, 2000; 166(8)∶628.
18.	Matzinger P. The danger model: a renewed sense of self.
19.	Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
20.	Murr MM, Yang J, Fier A, et al. Pancreatic elastase induces liver injury by activating cytokine production within Kupffer cells via nuclear factorKappa B [Ｊ]. J Gastrointest Surg, 2002; 6(3)∶474.
21.	Johnson GB, Brunn GJ, Platt JL. Cutting edge: an endogenous pathway to systemic inflammatory response syndrome (SIRS)like reactions through Tolllike receptor 4.
22.	Bradley EL 3rd. A clinically based classification system for acute pancreatitis. Summary of the International Symposium on Acute Pancreatitis, Atlanta, Ga, September 11 through 13, 1992.
23.	Fink GW, Norman JG. Intrapancreatic interleukin1beta gene expression by specific leukocyte populations during acute pancreatitis.
24.	Dugernier TL, Laterre PF, Wittebole X, et al. Compartmentalization of the inflammatory response during acute pancreatitis: correlation with local and systemic complications.
25.	Norman JG, Fink GW, Denham W, et al. Tissuespecific cytokine production during experimental acute pancreatitis. A probable mechanism for distant organ dysfunction.
26.	Denham W, Yang J, Fink G, et al. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis.
27.	Ikushima H, Nishida T, Takeda K, et al. Expression of Tolllike receptors 2 and 4 is downregulated after operation.
28.	Dybdahl B, Wahba A, Lien E, et al. Inflammatory response after open heart surgery: release of heatshock protein 70 and signaling through tolllike receptor4.
29.	Li Y, Zhou ZG, Xia QJ, et al. Tolllike receptor 4 detected in exocrine pancreas and the change of expression in ceruleininduced pancreatitis.
30.	Gotzinger P, Sautner T, Spittler A, et al. Severe acute pancreatitis causes alterations in HLADR and CD14 expression on peripheral blood monocytes independently of surgical treatment.

1. [Ｊ].Science, 2002; 296(5566)∶ 301.
2.
3.
4.
5. [Ｊ]. J Immunol， 2004; 172(1)∶20.
6.
7. [Ｊ]. Arch Surg, 1993; 128(5)∶586.
8.
9. [Ｊ]. J Surg Res, 1996; 63(1)∶369.
10. [Ｊ]. Am J Respir Crit Care Med, 2003; 168(2)∶148.
11. [Ｊ].Dig Dis Sci, 1997; 42(8)∶1783.
12.
13. [Ｊ].Gastroenterology, 1997; 113(5)∶1741.
14. [Ｊ]. Surgery, 2004; 135(4)∶376.
15. [Ｊ]. Circulation, 2002; 105(6)∶685.
16. [Ｊ]. Pancreas, 2005; 30(4)∶375.
17. [Ｊ]. Eur J Surg, 2000; 166(8)∶628.
18. Matzinger P. The danger model: a renewed sense of self.
19. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
20. Murr MM, Yang J, Fier A, et al. Pancreatic elastase induces liver injury by activating cytokine production within Kupffer cells via nuclear factorKappa B [Ｊ]. J Gastrointest Surg, 2002; 6(3)∶474.
21. Johnson GB, Brunn GJ, Platt JL. Cutting edge: an endogenous pathway to systemic inflammatory response syndrome (SIRS)like reactions through Tolllike receptor 4.
22. Bradley EL 3rd. A clinically based classification system for acute pancreatitis. Summary of the International Symposium on Acute Pancreatitis, Atlanta, Ga, September 11 through 13, 1992.
23. Fink GW, Norman JG. Intrapancreatic interleukin1beta gene expression by specific leukocyte populations during acute pancreatitis.
24. Dugernier TL, Laterre PF, Wittebole X, et al. Compartmentalization of the inflammatory response during acute pancreatitis: correlation with local and systemic complications.
25. Norman JG, Fink GW, Denham W, et al. Tissuespecific cytokine production during experimental acute pancreatitis. A probable mechanism for distant organ dysfunction.
26. Denham W, Yang J, Fink G, et al. Gene targeting demonstrates additive detrimental effects of interleukin 1 and tumor necrosis factor during pancreatitis.
27. Ikushima H, Nishida T, Takeda K, et al. Expression of Tolllike receptors 2 and 4 is downregulated after operation.
28. Dybdahl B, Wahba A, Lien E, et al. Inflammatory response after open heart surgery: release of heatshock protein 70 and signaling through tolllike receptor4.
29. Li Y, Zhou ZG, Xia QJ, et al. Tolllike receptor 4 detected in exocrine pancreas and the change of expression in ceruleininduced pancreatitis.
30. Gotzinger P, Sautner T, Spittler A, et al. Severe acute pancreatitis causes alterations in HLADR and CD14 expression on peripheral blood monocytes independently of surgical treatment.

《中國普外基礎與臨床雜志》

人急性胰腺炎早期外周血單核細胞Toll樣受體4表達變化

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

人急性胰腺炎早期外周血單核細胞Toll樣受體4表達變化

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料